Agilent offers cell analysis technology solutions that detect discrete changes in cellular bioenergetics in real-time. Real-time cell measurements provide a clear window into the critical functions driving cell signaling, proliferation, activation, toxicity and biosynthesis.
Agilent Seahorse XF Pro analyzer measures and reports the oxygen consumption rate (OCR), proton efflux rate (PER) or extracellular acidification rate (ECAR), as well as ATP production rates of live cells in a 96-well format. This analyzer features excellent OCR precision at low rates, verified instrument performance, optimized temperature control, and is automation enabled.
OCR, PER, and ATP production rates are critical measurements of energy metabolism and vital indicators of mitochondrial health, toxicity, glycolysis, and overall cellular (dys)function. Together, these measurements provide a systems-level view of cellular metabolic function in cultured cells and ex vivo samples.
The Agilent Seahorse XF T Cell Metabolic Profiling kit allows for robust and accurate measurements of both glycolytic and mitochondrial activities in T cell populations, providing a complete picture of T cell energy metabolism
The Agilent Seahorse XF Mito Tox Assay kit allows for the identification of drug-induced mitochondrial toxicity through direct functional measurements of mitochondrial oxygen consumption rates (OCR).
The Agilent Seahorse ATP Real-Time rate assay is the only assay that quantifies the rate of adenosine triphosphate (ATP) production from glycolysis and mitochondria simultaneously using label-free technology in live cells.
The Agilent Seahorse Cell Mito Stress Test kit is the widely recognized, well accepted standard assay for assessing mitochondrial function. Multiple parameters are obtained in this one assay including, basal respiration, ATP-linked respiration, maximal and reserve capacities, and non-mitochondrial respiration
The Agilent Seahorse XF Substrate Oxidation Stress Test Kits are a suite of optimized solutions for rapidly measuring cellular substrate oxidation by assessing changes in oxygen consumption (OCR) in live cells.
The Agilent Seahorse XF Palmitate Oxidation Stress Test Kit provides validated reagents to measure long chain fatty acid oxidation (FAO) via assessing changes in oxygen consumption (OCR) in intact cells.
The Agilent Seahorse Mito Fuel Flex Test kit provides an analytical method for measuring the basal state of mitochondrial fuel oxidation in live cells.
The Agilent Seahorse Glycolytic Rate Assay provides a precise measure of glycolysis in live cells, revealing transient responses and rapid metabolic switches not discernible in end-point lactate assays.
The Agilent Seahorse Glycolysis Stress Test kit measures the capacity of the glycolytic pathway after glucose starvation.
The Agilent Seahorse XF Plasma Membrane Permeabilizer (PMP) is a proprietary reagent that permeabilizes intact cells in culture. It selectively targets the cellular plasma membrane while leaving the mitochondrial membrane intact.
Agilent Seahorse XF Hu T Cell Activation Assay Kit measures human (Hu) T cell activation response within several minutes of stimulation using Seahorse XF Analyzers.